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   Design of TAL effector binding sites   

  
     

  
     
TALENdesigner genome browser
The genome browser provides convenient access to TALEN target sites in the coding region of the mouse, rat, and the human genome. For example, in the mouse genome 7.27 million identified target sites are available.
  
Scan sequences for binding and cleavage sites
Scan your own sequence to find potential TALEN binding and cleavage sites
  
TALEN target finder M
Genome-wide search for TALEN target sites
  
See the protocol for TALEN construction and the generation of knock-in and knockout mice in:
Wefers B, Panda S, Ortiz O, Brandl C, Hensler S, Hansen J, Wurst W, Kühn R (2013) Generation of targeted mouse mutants by embryo microinjection of TALEN mRNA. Nature Protocols 8(12), 2355-79.
  
Further reading:
Wefers B, Meyer M, Ortiz O, Hrabé de Angelis M, Hansen J, Wurst W, Kühn R (2013) Direct production of mouse disease models by embryo microinjection of TALENs and oligodeoxynucleotides. PNAS 110(10), 3782-87.